Abstract: 10.20 Multipotent Adult Progenitor Cells Support Lymphatic Growth In Wound Healing And Lymphedema

Results: Endothelial cells exhibited high viability (>90%) as measured via confocal microscopy using Hoechst 33342 and propidium iodide staining. Following 7 days of incubation, the necessary structural integrity of the endothelial monolayer lining the lumen was achieved. This was proven by the formation of a well-defined network of actinfilaments, stained with Alexa Fluor 488 ̈ phalloidin. Additionally, the diffusional permeability of this conduit was evaluated using fluorescent labeled dextran molecules. Future studies are focused on perfusing the lumen with media with a digital-control peristaltic pump.